Osmosis lab report

Diffusion and, osmosis experiments « The kitchen Pantry Scientist

Effects of physical and chemical stresses on membranes beet roots have large amounts of a red pigment called betacyanin, which is contained in the vacuole in healthy cells. We can therefore measure the integrity of the beet membranes by measuring the amount of betacyanin leakage. In this experiment we will measure the effects of temperature and organic solvents on membrane integrity by mistreating beet roots and measuring the leakage of betacyanin into the medium. Cut a few cores of beet root using a cork borer. Prepare 10 cylinders 2 cm long from these cores. Rinse the cylinders in tap water to wash off betacyanin from injured cells Place 4 cylinders in 3 mls distilled water in test tubes labeled 1-4. Leave 1 on your bench top for 20 minutes. Leave 2 in a 40_ C water bath for 20 minutes.

Osmosis in animal cells. Prepare a wet mount of sheep blood by diluting a small amount with 150 mm nacl. Examine under your homework microscope, first with the essays 4x, then 10x, then 40x objectives, then draw a few cells in the third of the circle labeled "a." Now prepare a second wet mount by diluting a small amount of blood with distilled water. Act quickly, and watch what happens with your microscope. Once you've seen a few cells blow up, draw a few of the hemolyzed cells in the third labeled "b." Now prepare a third wet mount by diluting a small amount of blood with 300 mm nacl, and observe under your microscope. Once you've seen some cells shrivel, draw them in the third labeled "c and enter the appearance of the cells in the table on your data sheet. Give 3 or 4 sentences of introduction (but no more!) in which you explain: 1) what hydrostatic pressure is, 2) why it is important for animal cells to be isoosmotic with their environment, and 3) the specific objectives of this experiment. Under "Results" you should include a sentence or two describing why you performed your experiments, a sentence explaining your table and your figure, and a sentence or two summarizing the key results. Finally, your Discussion should: 1) compare the appearance of the three groups of cells, 2) explain why the cells hemolyzed in distilled water, 3) explain why the cells shriveled in 300 mm nacl, and, 4) compare the effects of distilled water on Elodea and red. Why didn't Elodea cells burst in distilled water?

osmosis lab report

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Now examine the leaves in drops of 150, 300, 5 mm nacl. Enter whether the cells look turgid or plasmolysed after 15 minutes (i.e. After you finished your first drawing) in your data sheet. You should find that in at least one of these samples the cells have plasmolysed, and all the chloroplasts are concentrated in one portion of the cell. Draw a few of these cells on the half of the circle labeled "b.". Give 3 or 4 sentences of introduction (but no more!) in which you explain: 1) what turgor is, 2) why it is important for plant cells, and 3) the specific objectives of this experiment. "Results" should include a sentence or two describing why you performed your experiments, twist a sentence explaining your table and your figure, and a sentence or two summarizing the key results. Finally, your Discussion should: 1) explain the difference in appearance of the normal and plasmolysed cells, 2) explain why the cells plasmolysed in high salt concentrations, and 3) estimate the salt concentration which is isoosomotic with the.

osmosis lab report

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In older cells, most of the internal volume is occupied by a watery sac called the vacuole. In these cells the cytoplasm, which contains the chloroplasts, is restricted to the periphery of the cell (just inside the plasmalemma). No chloroplasts can pass into the vacuole, because it is bound by a membrane called the tonoplast. You should be able to make out the vacuole by using the fine-focus adjustment knob on your microscope to focus through each cell. As you examine the. Elodea cells, you might notice that the chloroplasts of some seem to move in a cyclical motion. That movement is called cytoplasmic streaming, and is an important means by which material is moved within the cells. Sketch a few, elodea cells on the half labeled "a" of the circle on your datasheet, showing the proper shape and proportion relative to the width of field. Label all structures indicated strange above, and provide scaling.

You should be able to see individual cells clearly. Notice the small green disc-like structures within each cell. These are the chloroplasts, the site of photosynthesis. Also note the rigid cell walls surrounding each cell. Cell walls are mesh-like rather than solid structures, which allows us to see the internal contents of the cells. The cell walls of adjacent cells join at an area called the middle lamella. The plasmalemma, or cell membrane, lies immediately inside the cell wall.

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osmosis lab report

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Leave the bags for two hours, then remove them, blot the outsides dry and weigh them again. Record the weights and their colors on your data sheet. Give 3 or 4 sentences of introduction (but no more!) in which you explain: 1) what osmosis is, 2) why it is important for cells, and 3) the specific objectives of this experiment. Under "Results" you should include a sentence or two describing why you performed your experiments, a sentence explaining your table, and a sentence or two summarizing the key results. Finally, your "Discussion" should interpret your results, explaining for each bag why it gained or lost weight and why it did or did not change color (be brief, no more than five or six sentences).

Osmosis in plant cells, elodea is an aquatic plant used in aquaria. It is ideal for viewing the structure of plant cells essay because the leaves are very thin. Elodea are found in a large beaker on the side table. Prepare five wet mounts: for each, pluck a leaf from near the tip of one of the shoots, then place it on a clean glass microscope slide. Then add drops of distilled water, 150, 300, 500 or 1000 mM NaCl, respectively, and place a cover slip on the leaf. First examine the sample in distilled water under your microscope, rights starting with the 4x, then 10x, then 40x objectives.

"The largest halo developed around Lugol's Iodine. Although much of this seems redundant, remember that scientists frequently only read part of a paper rather than the whole thing, so each section needs to be self-explanatory. For the same reason, you must give your table a title and a brief caption explaining what is presented (e.g.: "Table I: diffusion rates of various materials through.5 agar. Substances listed in table i were placed in wells formed.5 agar, and diameters of the halos formed after one hour were measured. finally, your Discussion should interpret your results, explaining why each substance formed a different size halo (be brief, no more than five or six sentences). Osmosis in a model system.


Obtain 4 dialysis bags (a material which is freely permeable to water and small molecules, but not to larger molecules). Fill one with water, fold over the top, squeeze out all the air (its. To squeeze out a bit of solution as well then tie the bag. The bag should be limp. Blot the outside of the bag dry, then weigh it and record it on your data sheet. Repeat this process with three more bags, except fill the second with 25 glucose, the third with 1 starch, and the fourth with litmus. After weighing them, place the bags of glucose and starch in a beaker containing 20 mls of water, then add 3 drops of Lugols iodine. Place the bags containing water and litmus in a beaker containing 20 mls of pH 9 buffer.

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Do not overfill the essay wells! Measure the diameter of the halo formed by each substance after two hours, and enter in the table in the "Results" section of your data sheet. Report your experiment on your datasheet. Give 3 or 4 sentences of introduction (but no more!) in which you explain: 1) what diffusion is, 2) why it is important for cells, and 3) the specific objectives of this experiment. Under "Materials and Methods" concisely describe your methods using past tense. "Results" should include a sentence or two describing why you performed your experiments, (e.g. "We wished to study the relationship between the molecular weight of a substance and the rate at which it diffuses. We therefore measured the diffusion rate through agar of the materials listed in Table. a sentence explaining pdf your table (e.g., "results of this experiment are presented in Table i and a sentence or two summarizing the key results (e.g.

osmosis lab report

increase in internal osmotic pressure. Conversely, if a vesicle is placed in a solution containing high concentrations of a chemical which cannot cross the membrane, the concentration of water will be lower outside. This vesicle is hypoosmotic to its surroundings and water will leave by osmosis. The second part of this weeks lab will demonstrate osmosis in a model system and in living tissues. For membranes to function properly the lipid bilayer must be intact, and in the final part of this weeks lab we will demonstrate this by subjecting membranes to various insults which affect the lipid bilayer and then seeing how this affects the integrity of the. This week's activities, today's lab has three purposes: 1) to study diffusion, 2) to study osmosis in model systems and in plant and animal cells, and 3) to study the effects of various stresses on membranes. Additional information about these processes can be found in Chapter 5 of your text. Obtain a petri dish with.5 agar in which four wells have been formed. Fill one well with Lugols Iodine (FW 166 a second with Malachite Green (FW 346.5 a third with Acid Fuchsin (FW 579.6) and the final well with Brilliant Blue g (FW 854).

Substances move in and out of cells either by diffusion, or by active transport. Diffusion is the net movement of chemicals to regions where their concentrations are lower as a result of random motions. Diffusion occurs in order to decrease the free energy of a system (the technical definition is that it occurs in order to maximize entropy). Since diffusion occurs as a result of random motion, the rate at which a particular molecule diffuses is inversely proportional to its molecular mass: the larger it is, the slower it moves. The first part of this weeks lab will demonstrate this. Each chemical diffuses along its own concentration gradient independently of the other chemicals present; because of this, funny things can happen when some molecules can freely pass mba across membranes whereas others can not. In particular, osmosis is the diffusion of water across a membrane freely permeable to water but not to other chemicals. If certain molecules are trapped inside a vesicle placed in water, the concentration of water inside the vesicle will be lower than that of the surroundings.

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Lab 4 instructions, bIO 121 lab instructions, lab 4: Membranes. Introduction, membranes form the barriers separating cells from their surroundings, or one compartment of a cell from another. In addition to forming a barrier keeping desired materials inside and unwanted materials outside, they must make also permit the controlled import and export of specific chemicals. Moreover, membranes must be able to pinch off or create vesicles. Thus, membranes must fulfill many criteria. Living organisms construct membranes capable of performing these functions from a mixture of lipids and proteins, where the lipids form a bilayer in which the proteins are embedded. The lipid bilayer acts as a barrier to polar molecules because the core of the bilayer is made of hydrophobic fatty acids; however, the fluidity of these fatty acids permits the membrane to remain pliable and fuse with other membranes. Proteins embedded in the membrane perform a variety of functions; some transport polar molecules across the membrane, others transmit information, a third group attaches the membrane to the cytoskeleton, while others perform many other functions.


osmosis lab report
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